Chemical Safety Science, 2017, Volume 1, No 1, p. 146 — 157
Protective methods and materials
УДК 619:616-085.33:542 
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DOI: 10.25514/CHS.2017.1.11440
ANTIVIRAL ACTIVITY AND THERAPEUTIC AND PREVENTIVE EFFECT OF PHOSPHONOACETIC ACID AND ITS DERIVATIVES
M. M. Zubairov1, Yu. O. Selyaninov1*, A. V. Roshchin2, and P. S. Khokhlov2
1State Science Institution National Research Institute of Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences, Volginsky, Vladimir region, Russia
2Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia
Received April 20, 2017
Published June 26, 2017
Abstract – Antiviral (virustatic) activity of 127 derivatives of phosphonoacetic acid was examined in porcine bone marrow cell culture; consequently, 16 of them were selected and tested for studying their therapeutic and prophylactic effect. Analysis of combined data on their synthesis efficiency, reproducibility, and therapeutic activity against African swine fever (ASF) resulted in choosing three compounds, i.e. phosphonoacetic acid, phosphonoacetic acid complex with 7-amino-1,3,5-triazaadamantane, and potassium pyridine salt of phosphonoacetic acid, all of which prevented mortality of more than 80% of infected animals versus 100% mortality of animals in control group. The use of phosphonoacetic acid in combination with metisazone under microepizootic conditions prevented mortality of all piglets contacted with the diseased ones, in comparison to 100% mortality in untreated animals. A possibility for virus carriage removal under ASF was shown (dosages, terms and frequency of chemotherapeutic agent administration were determined). The possibility of obtaining type-specific sera was established which left out the step of attenuation of virulent strains. The result allowed to shorten the serum preparation time by 3-12 times which might be crucial for serotyping the virus, since attenuation of individual strains can take up to 6-12 months or more. The results contribute to developing recommendations and measures against ASF.
Keywords: antiviral activity, virustatic action, therapeutic and preventive effect, maximum tolerated dose, minimum effective dose, chemotherapeutic index, African swine fever, phosphonoacetic acid, metisazone.