Chemical Safety Science, 2017, Volume 1, No 1, p. 226 — 237

 

Chemical hazard sources. Hazardous chemical substances

 

UDC [632.951.2: 54.064: 543.05]+ 543.544.5.068.7                       Download PDF (RUS)

DOI: 10.25514/CHS.2017.1.11447

 

NEW PROCEDURE FOR DETERMINATION OF BUTENOLIDE INSECTICIDE FLUPYRADIFURONE IN SOIL BY MEANS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH UV DETECTION

A. A. Kuzovkova⃰  and L. S. Ivashkevich

Republican Unitary Enterprise “Scientific Practical Centre of Hygiene», Minsk, Republic of Belarus

Received April 20, 2017

Published June 26, 2017

Abstract – A new procedure for flupyradifurone determination in soil by high-performance liquid chromatography with UV detection was been developed. A 5-micron Eclipse XDB-C18 (Agilent) analytic column (250 x 4.6 mm i.d.) was used as a stationary phase. A mixture of solution A (methanol: 10 mmol aqueous ammonium formiate : formic acid = 100 : 900 : 0.12 (v : v : v)) and solution B (methanol: 10 mmol aqueous ammonium formiate: formic acid = 900: 100: 0.12 (v : v :v)) was used as a mobile phase. Gradient separation at a flow rate of 0.5 cm³/min was applied. The compound identification was carried out by using retention time value at 260 nm. Quantification was performed using absolute calibration method. Flupyradifurone extraction was conducted with deionized water under sonication. Extract was analyzed without further purification. The flupyradifurone recovery in soil amounted for 53.7% for the concentration range 0.5–5 mg/kg. The limit of quantitative detection was found to be 0.5 mg/kg.

Keywords: flupyradifurone, soil, high-performance liquid chromatography (HPLC), UV detection.