Chemical Safety Science, 2018, Volume 2, Issue 1, p. 191 — 205

 

Utilization and biodegradation of wastes

UDC  579.695; 546.85; 502.55; 661.63                                        Download PDF (Rus)

DOI: 10.25514/CHS.2018.1.12893

 

 

EFFECT OF SELECTION UPON RESISTANCE OF MICROORGANISMS TO WHITE PHOSPHORUS

A. Z. Mindubaev¹*, A. D. Voloshina¹, N. V. Kulik¹, Sh. Z. Validov², A. Saparmyradov², S. T. Minzanova¹, L. G. Mironova¹, and D. G. Yakhvarov¹

¹State Budgetary-Funded Institution of Science Arbuzov Institute of Organic and Physical Chemistry of Kazan Scientific Center, Russian Academy of Sciences, Kazan’, Russia
²Kazan (Volga region) Federal university, Kazan’, Russia

Received December 26, 2017

Published June 25, 2018

Abstract – Aiming at detoxification of dangerous technogenic pollutant – white phosphorus – an effect of controlled selection of a series of microorganisms (Aspergillus niger AM1, Streptomyces sp. A8, Trichoderma asperellum F-1087) on their resistance to white phosphorus was studied in synthetic culture media containing P₄ as the sole phosphorus source at concentration level of up to 1%. The selection resulted in increasing resistance of the studied organisms. The best adaptability to white phosphorus was demonstrated by streptomycete strain Streptomyces sp. A8, which was further investigated using biochemical analysis and metabolic profiling. A procedure for sterilizing P₄ with acetone treatment under mild conditions was developed. Practical recommendations for neutralizing soil contaminated with white phosphorus were proposed, as exemplified by soil treatment with a powdered formulation of the fungus T. asperellum F-1087.

Keywords: detoxication, white phosphorus, culture media, Aspergillus niger АМ1, Streptomyces sp. А8, Trichoderma asperellum F-1087.