Chemical Safety Science, 2018, Volume 2, No 2, p. 119 — 130


Identification of hazardous substances


UDC 543.48 + 577.1                                                                   Download PDF (RUS) 

DOI: 10.25514/CHS.2018.2.14109



 A. V. Lobanov1,2*, I. A. Nagovitsyn1,3, and G. K. Chudinova3 

1Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

2Moscow State Pedagogical University, Institute of Biology and Chemistry, Moscow, Russia

3Prokhorov Institute of General Physics, Russian Academy of Sciences, Natural Sciences Center, Moscow, Russia 

Received October 02, 2018

Published December 26, 2018 

Abstract – A potentially useful procedure for determining albumin in the aqueous media by immunosensor technique with a fluorescent type of detection has been described. The registration principle is based on energy transfer between two fluorescent molecules immobilized on antigen and antibody interacting with each other. The sensor element is implemented in a solid-phase version using the Langmuir-Blodgett technology. The detection limit for albumin in solution makes up 10-11 M with the optimal time for analysis in the range of 6-8 minutes. 

Keywords: albumins, immunosensors, fluorescence, energy transfer, thin films.


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